Volume 4 : Issue 3, September 2014

Table of Contents,

Issue 3 (25 September 2014), pp. 56-74

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pii: S2322455X1400009-4

Effects of Mannan Oligosaccharide and Saccharomyces cerevisiae on Gut Morphology of Broiler Chickens
JWPR-1004
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Original Article, D9
Pani Padihari V., Prasad Tiwari S., Sahu T., Kumar Gendley M. and Kumar Naik S.
J. World's Poult. Res.
4(3): 56-59, 2014

ABSTRACT: 150 day old Vencobb broiler chicks were randomly allocated to 5 treatment groups with 3 replicates of 10 chicks in each to determine the effect of mannan oligosaccharide (MOS) and Saccharomyces cerevisiae in gut morphology of broilers. The trial lasted for 6 weeks. For microscopic examination the representative samples of each segment of intestine were collected and fixed in the 10% buffered formalin.  No significant difference was observed in treatments at length of different segments of intestine and villus height/crypt depth ratio. Significant (P< 0.05) difference observed for the mean height of the duodenum, jejunum, and ileum villus amongst different dietary treatments, being highest in T5 and lowest in T2. Mean crypt depth of the duodenum and ileum villus also differed significantly (P< 0.05) amongst treatments. The highest mean crypt depth in the duodenum was recorded in T5 and lowest in T2 whereas the highest mean crypt depth of caecum was recorded in T3 and the lowest in T1. Thickness of tunica muscularis was significantly (P< 0.05) decreased in all segments of intestine except colorectum as MOS and S. cerevisae added to the diet. Height of the epithelium of villi differed significantly (P< 0.05) amongst treatments in all segments of intestine except caecum being maximum in T5 and minimum in T2. It was concluded that supplementation of MOS and S. cerevisiae improves the gut health of broiler chickens.
Keywords: Broilers, Gut, Mannan oligosaccharide, Saccharomyces cerevisiae

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pii: S2322455X1400010-4

Evaluation Protein Digestibility, Metabolic Energy of Autoclaved Komak Beans (Lablab purpureus L sweet) on Broiler

Original Article, D10
Ayuning Tyas DW, Sjofjan O and Eka Radiati L.
J. World's Poult. Res.
4(3): 60-63, 2014

ABSTRACT: This research was aimed to evaluate protein digestibility and metabolic energy of autoclaved komak beans on broilers. The material used consisted of basal feed, komak bean, local soybean, imported soybean, twenty thirty-seven-day broilers, and metabolic henhouse. The methods in the research implemented completely randomized design with five treatments and four replications (Lablab Purpureus or LP0: basal diet; LP1: 15% local soybean; LP2: 15% imported soybean; LP3: 15% komak beans; LP4: 15% autoclaved of komak beans). The variables observed in this research were the protein digestibility, Nitrogen retention, Apparent Metabolic Energy, and Apparent Metabolic Energy corrected Nitrogen. The results showed that protein digestibility, nitrogen retention, AME and AMEN of autoclaved komak beans were not significant (P>0.05) on 37 days old broilers. It concluded that the protein digestibility, nitrogen retention, AME and AMEn in autoclaved komak beans had the same effectiveness as soybean.
Key words: Autoclave, Broiler, Lablab purpureus, Metabolic Energy, Protein Digestibility

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pii: S2322455X1400011-4

Pathogenicity Assessment of Seven Variants of Infectious Bronchitis Virus Isolated from Commercial Broiler Chickens during 2013 in Egypt



Original Article, D11
Sediek M.E. and Awad A.M.
J. World's Poult. Res.
4(3): 64-74, 2014

ABSTRACT: The aim of this study was to evaluate the pathogenicity of seven variants of IBV isolates (Coded, 11, 15, 21, 13, 19, 22, 24) using commercial broiler chickens. These isolates isolated from 25 commercial broiler flocks raised in El-Behera and Kafr-Elsheikh governorates in 2013, and were identified by the AGPT against reference IBV Beaudette antiserum and RT-PCR and characterized by partial sequence analysis of S1 gene as variant 2 IBV strains. The results at 2 weeks of PI revealed that all isolates were able to induce serological response and respiratory signs in the form of conjunctivitis, mainly of a frothy type, associated with lacrimation, edema and cellulitis of the periorbital tissues, sneezing, coughing, tracheal rales and gasping were observed in all infected groups in a varying degrees and varying times of appearance and disappearance. In addition the birds appeared lethargic, reluctant to move. No mortalities were recorded during the course of experiment in all groups. The main lesions of PI were slight increase of mucin, congestion and presence of nasal exudate in the trachea and small patches of pneumonia in chickens challenged with 4 IBV isolates only (NO. 15, 21, 22, 24). Renal lesions (nephritis and enlarged kidney) which were induced in chickens challenged with 3 isolates (NO. 15, 21 and 24) were not as pronounced as seen in the field. Histopathological finding of PI, exhibited tracheal lesions with inflammatory cells infiltration in the lamina propria and submucosa, edema in the submucosa, desquamation of the lining epithelial of the mucosa and activation of goblet cells. In addition to renal lesions with interstitial nephritis which characterized by infiltration of inflammatory cells (heterophils, lymphocytes and plasma cells) in the interstitial tissue.
Keywords: Pathogenicity, Assessment, Variants, Infectious Bronchitis Virus, Broiler.

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Original Article, D11
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J. World's Poult. Res.
4(3): , 2014

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